SPME: New Developments and Pharmaceutical Applications


Solid-phase microextraction (SPME) is a simple, fast, sensitive, and environmentally-friendly sample preparation technique that integrates sampling and sample preparation steps. This course will cover the main principles of SPME, calibration methods, coupling strategies of SPME to GC, LC as well as method development strategy.  Applications of SPME in the fields such as forensic, environmental, food and beverage and clinical analysis will be discussed. Advances in SPME will be highlighted including: ligand-receptor binding and plasma protein binding studies, in vivo sampling of freely moving animals for pharmacokinetic and metabolomic studies, medical applications, automation in high-throughput format and direct tissue analysis.


The course is targeted at new and current SPME users. It will be of interest to analytical chemists, laboratory supervisors, scientists and industry regulators in the environmental, food and beverage, pharmaceutical, clinical, cosmetic and other fields. The features of in vivo chemical biopsy SPME sampling will be of particular interest to biologists and life scientists as well as clinical chemists.


  • Introduction to SPME
  • Theoretical principles of SPME.
  • SPME interfaces
  • Calibration of SPME
  • SPME method development
  • Automation of SPME
  • In vivo sampling using SPME
  • Drug and clinical analysis
  • Medical applications
  • Food, flavor and fragrance analysis
  • Environmental analysis


Dr. Janusz Pawliszyn is the inventor of SPME and a professor in the Department of Chemistry, University of Waterloo, Canada. He is the author of over 700 journal publications and 600 invited/plenary lectures. He has received several prestigious awards for his scientific achievements, and SPME is considered one of the six “Great Ideas of a Decade” by ACS Analytical Chemistry journal. He presently holds the Canada Research Chair and his IF is 103.

Dr. Barbara Bojko is a head of the Department of Pharmacodynamics and Molecular Pharmacology at Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Poland. Her research is focused on biomedical applications of SPME, especially on low- and non-invasive tissue analysis in the field of oncology, transplantology, and translational medicine. Her  research team also works on application of uniform microextraction-based protocols at subsequent steps of drug discovery and development: from high throughput format compatible with routinely used 2D and 3D cell line culturing assays to minimum invasive in vivo samplings of laboratory animals.

Dr. Ezel Boyaci is an associate professor at Department of Chemistry, Middle East Technical University, Turkey. She obtained PhD at the Izmir Institute of Technology, Turkey and spent 5 years researching at Prof. Dr. Janusz Pawliszyn’s Research Group at University of Waterloo. Her research interests focused on clinical, pharmaceutical and environmental applications of various formats of solid phase microextraction (SPME), development of new extractive phases for SPME and metabolomics studies using SPME with complementary instrumental techniques such as LC-MS/MS, GC-MS, and LC-HRMS.

Dr. Wei Zhou is a postdoctoral fellow in Professor Janusz Pawliszyn’s research group, University of Waterloo, Canada. He obtained his bachelor’s degree in biopharmaceuticals and Ph.D. degree in pharmaceutical science in Wuhan University, China. His research is focused on SPME and MS based techniques including SPME-MS direct coupling, high-throughput and automated SPME work flow, CE/CEC-MS and ambient MS.