Talk Title: Analytical strategies for Extracellular Vesicles characterization in therapeutic and diagnostic applications
Caterina Temporini was born on 20 April 1976 and graduated in Pharmacy at the University of Pavia in 2000: She obtained her PhD from the same institution in 2005. Since 2015 she is Associate Professor in Pharmaceutical Analysis at the University of Pavia.
Her main research deal with the development and application of liquid phase separations and mass spectrometry (LC-MS) based methods for the functional and structural analysis of proteins.
The earliest activities focused on the development of enzyme-based chromatographic stationary phases for in-flow reactions in different pharmaceutical applications, ranging from enantioselective discrimination/catalysis (penicillin G acylase, lipase), peptide/glycopeptide mapping (trypsin, chymotrypsin, pronase), recombinant protein production (enterokinase), inhibition studies (purine nucleoside phosphorylase as target for antitubercular drugs). Biochromatographic systems based on immobilized receptors, such as membrane (GPR17 and A2A) and soluble (PPAR-α and PPAR-) receptors, were also developed to characterize ligand-protein interactions with a great impact in the discovery of new potential ligands.
In the recent years, the research carried out and directed by Prof. Temporini is strongly dedicated to the development and the application of integrated analytical platforms for structural analysis and process optimization of biopharmaceuticals, including polypeptides, glycopeptides, and monoclonal antibodies from different sources (CHO cells, plants). The characterization of recombinant protein critical quality attributes is obtained by the most recent chromatographic stationary phases (HILIC, SEC, RP, IEX, Affinity) and instrumentations (HRMS). Within a multidisciplinary network, she is also working on the design, the preparation and analytical characterization of neo-glycocojugate proteins as potential vaccines active against tuberculosis.